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  • Title: Can hTNF-alpha be successfully produced and secreted in filamentous fungus Aspergillus niger?
    Author: Krasevec N, van den Hondel CA, Komel R.
    Journal: Pflugers Arch; 2000; 439(3 Suppl):R84-6. PubMed ID: 10653151.
    Abstract:
    A gene-fusion expression strategy was applied for the heterologous expression of hTNF-alpha in A. niger AB1.13. The TNF-alpha gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not present in the culture medium. From the expression of two hTNF-alpha analogues, LK 811 (Cys95/148) and LK 802 (Cys95/148, His107/108) respectively, we concluded that oligomerisation was not the critical point for secretion of hTNF-alpha in A. niger, but more probably improper folding already at the stage of monomer formation, or even incorrect processing of the molecule during the secretion pathway.
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