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  • Title: Cell surface display of hepatitis B virus surface antigen by using Pseudomonas syringae ice nucleation protein.
    Author: Kim EJ, Yoo SK.
    Journal: Lett Appl Microbiol; 1999 Nov; 29(5):292-7. PubMed ID: 10664968.
    Abstract:
    A new system designed for cell surface display of recombinant proteins on Escherichia coli was evaluated for expression of eukaryotic viral antigens. The major surface antigen of hepatitis B virus (HBsAg) was fused to the ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae. Western blotting, immunofluorescence microscopy, whole-cell ELISA, and ice nucleation activity assay confirmed expression of recombinant proteins on the surface of Escherichia coli. This study indicated that INP-based cell surface display can be used for epitope mapping and recombinant bacteria expressing hepatitis viral antigens may be used for developing live vaccines.
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