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  • Title: A reversed-phase high-performance liquid chromatographic method to analyze retinal isomers.
    Author: Cia D, Bonhomme B, Azim M, Wada A, Doly M, Azaïs-Braesco W.
    Journal: J Chromatogr A; 1999 Dec 24; 864(2):257-62. PubMed ID: 10669293.
    Abstract:
    A high-performance liquid chromatographic (HPLC) procedure was developed to separate all-trans-, 13-cis-, 11-cis- and 9-cis-retinal isomers. Two reversed-phase Vydac C18 columns in series were used with an isocratic solvent system of 0.1 M ammonium acetate-acetonitrile (40:60, v/v) as mobile phase and all-trans-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl-2,4,6,8-no natetraene-1-ol (TMMP) as internal standard. Prior to HPLC, the retinal isomers were efficiently extracted in their original isomeric conformation using dichloromethane-n-hexane in the presence of formaldehyde. This technique is suitable for the assay of 11-cis- and all-trans-retinal isomers in retina.
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