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  • Title: N-Glycan structures of an osteopontin from human bone.
    Author: Masuda K, Takahashi N, Tsukamoto Y, Honma H, Kohri K.
    Journal: Biochem Biophys Res Commun; 2000 Feb 24; 268(3):814-7. PubMed ID: 10679288.
    Abstract:
    N-Glycan structures of osteopontin (a bone matrix protein) from human bone (lumbar vertabrate) are reported in detail. Asn-linked glycan portion was released from 100 microg of osteopontin by digestion with glycoamidase A (from sweet almond), and the reducing ends of the N-glycans were reductively aminated with 2-aminopyridine. The derivatized N-glycans were separated and structurally identified by a multidimensional mapping technique on HPLC columns. Two major N-glycan structures were also confirmed by mass spectrometry. The proposed structures are shown below. The result should permit future comparison with the N-glycan structures of osteopontins obtained from other sources (kidney tissues, macrophages, urinary stones, human milk, etc.).
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