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  • Title: Affinity chromatography of endoglucanase of Trichoderma viride by concanavalin A-agarose.
    Author: Gong CS, Chen LF, Tsao GT.
    Journal: Biotechnol Bioeng; 1979 Feb; 21(2):167-71. PubMed ID: 106904.
    Abstract:
    Endoglucanase (C kappa cellulase) and cellobiase are often cross-contaminated in separation procedures by ion-exchange chromatography such as DEAE-cellulose. By using concanavalian A (Con A)-agarose chromatography, C kappa cellulase and cellobiase from Trichoderma virde can be separated. C kappa cellulase showed affinity toward Con A. indicating a glycoprotein containing alpha-D-mannopyransyl and alpha-D-glucopyranosyl end groups or internal 2-O-D-mannopyranosyl residues in sugar moieties. This method provides a way to estimate the quantities of C kappa enzyme produced by T. viride and possibly by other organisms.
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