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  • Title: Penicillin G production by immobilized whole cells of Penicillium chrysogenum.
    Author: Morikawa Y, Karube I, Suzuki S.
    Journal: Biotechnol Bioeng; 1979 Feb; 21(2):261-70. PubMed ID: 106905.
    Abstract:
    Penicillium chrysogenum was immobilized in polyacrylamide gel prepared from 5% acrylamide monomers (85% acrylamide and 15% N,N'-methylene bisacrylamide). Penicillin produced from glucose by the immobilized mycelium was 17% of that produced by washed mycelium. However, the activity of penicillin production of the washed mycelium decreased with repeated use. On the other hand, the activity of the immobilized mycelium increased initially and decreased gradually with repeated use. The rate of oxygen uptake of the immobilized mycelium was about 30% of that of the washed mycelium. The immobilized mycelium required oxygen for the production of penicillin.
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