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  • Title: Leptin regulation of prepro-orexin and orexin receptor mRNA levels in the hypothalamus.
    Author: López M, Seoane L, García MC, Lago F, Casanueva FF, Señarís R, Diéguez C.
    Journal: Biochem Biophys Res Commun; 2000 Mar 05; 269(1):41-5. PubMed ID: 10694474.
    Abstract:
    The aim of this study was to determine the effects of leptin treatment on prepro-orexin and orexin receptor expression in the rat hypothalamus. Adult male rats, food-deprived for 48 and 72 h, were treated one time with vehicle or leptin (10 microg, icv). Prepro-orexin mRNA content was measured by semiquantitative RT-PCR, Northern blot, and in situ hybridization; orexin receptor 1 and 2 mRNA content was quantified by Northern blot and/or semiquantitative RT-PCR. Our results indicate that leptin inhibits a fasting-induced increase in prepro-orexin mRNA and orexin receptor 1 mRNA levels in the rat hypothalamus, while orexin receptor 2 mRNA levels were unchanged in all situations evaluated. These data provide direct evidence for an additional mechanism of adaptation of the hypothalamus to food deprivation and for a new effect of leptin in the regulation of food intake.
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