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  • Title: Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin.
    Author: Kisiel D, Gindzieński A, Gadek A.
    Journal: Acta Biochim Pol; 1999; 46(3):753-7. PubMed ID: 10698283.
    Abstract:
    O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.
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