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  • Title: Reversal of insulin-dependent diabetes using islets generated in vitro from pancreatic stem cells.
    Author: Ramiya VK, Maraist M, Arfors KE, Schatz DA, Peck AB, Cornelius JG.
    Journal: Nat Med; 2000 Mar; 6(3):278-82. PubMed ID: 10700229.
    Abstract:
    Ductal structures of the adult pancreas contain stem cells that differentiate into islets of Langerhans. Here, we grew pancreatic ductal epithelial cells isolated from prediabetic adult non-obese diabetic mice in long-term cultures, where they were induced to produce functioning islets containing alpha, beta and delta cells. These in vitro-generated islets showed temporal changes in mRNA transcripts for islet cell-associated differentiation markers, responded in vitro to glucose challenge, and reversed insulin-dependent diabetes after being implanted into diabetic non-obese diabetic mice. The ability to control growth and differentiation of islet stem cells provides an abundant islet source for beta-cell reconstitution in type I diabetes.
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