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  • Title: Solid phase extraction of clenbuterol from plasma using immunoaffinity followed by HPLC.
    Author: Rashid BA, Kwasowski P, Stevenson D.
    Journal: J Pharm Biomed Anal; 1999 Nov; 21(3):635-9. PubMed ID: 10701431.
    Abstract:
    An immuno-extraction column for clenbuterol has been prepared. Optimum conditions for the selective retention and elution of clenbuterol have been developed, based on a modification of our earlier work on morphine, chlortoluron and isoproturon. Clenbuterol could be retained on the immuno-column then eluted in one x one ml fraction using 50% methanol in phosphate buffered saline pH 2. On columns containing antisera (but not to clenbuterol) the clenbuterol was removed in the washing step. HPLC-UV determination gave clean traces. Day-to-day reproducibility was improved by precipitating the plasma proteins with acetonitrile.
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