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  • Title: Isolation and characterization of high and low differentiation-inducible Friend leukemia lines.
    Author: Ikawa Y, Aida M, Inoue Y.
    Journal: Gan; 1976 Oct; 67(5):767-70. PubMed ID: 1071084.
    Abstract:
    Induction of erythrodifferentiation was carried out by addition of dimenthyl sulfoxide or butyric acid to the culture medium among 80 subclones of T-3-Cl-2 Friend mouse leukemia line. Hemoglobin-positive cells were counted on day 5 of the inducer treatment on the hemocytometer after benzidine staining. Some cell lines were indicible by both of the inducers, but others were insensitive to dimethyl sulfoxide, but sensitive to butyric acid. Eight characteristic T-3-Cl-2 subclones have been selected; 2 highly inducible, 2 moderately inducible, and 4 minimally inducible. The data of differentiation inducibility together with the history of the T-3-Cl-2 line may facilitate extensive application of the T-3-Cl-2 system.
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