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  • Title: Isolation of U(VI) reduction-deficient mutants of Shewanella putrefaciens.
    Author: Wade R, DiChristina TJ.
    Journal: FEMS Microbiol Lett; 2000 Mar 15; 184(2):143-8. PubMed ID: 10713412.
    Abstract:
    A U(VI) reduction-deficient mutant (Urr) screening technique was developed and combined with chemical mutagenesis procedures to identify a Urr mutant of Shewanella putrefaciens strain 200. The Urr mutant lacked the ability to grow anaerobically on U(VI) and NO(2)(-), yet retained the ability to grow anaerobically on eight other compounds as terminal electron acceptor. All 11 members of previously isolated sets of Fe(III) and Mn(IV) reduction-deficient mutants of S. putrefaciens 200 displayed Urr-positive phenotypes with the Urr screen and were capable of anaerobic growth on U(VI). This is the first reported isolation of a respiratory mutant that is unable to grow anaerobically on U(VI) as terminal electron acceptor.
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