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  • Title: Development of an affinity-driven cross-linker: isolation of a vitamin D receptor associated factor.
    Author: Swamy N, Addo JK, Ray R.
    Journal: Bioorg Med Chem Lett; 2000 Feb 21; 10(4):361-4. PubMed ID: 10714500.
    Abstract:
    A vitamin D analogue containing an affinity and a photoaffinity probe (affinity-driven cross-linker, Double Label) was synthesized. An unknown factor, associated with vitamin D receptor (VDR), was isolated from rat liver nuclear extract using a GST-VDR-ligand-binding domain fusion protein (GST-VDR-LBD), affinity labeled with Double Label, and protein-protein cross-linking by photolysis.
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