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  • Title: Quantitation of nifedipine in human plasma by on-line solid-phase extraction and high-performance liquid chromatography.
    Author: Yriti M, Parra P, Iglesias E, Barbanoj JM.
    Journal: J Chromatogr A; 2000 Feb 18; 870(1-2):115-9. PubMed ID: 10722068.
    Abstract:
    An analytical methodology for nifedipine quantitation in plasma by on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contain C2 and the analytes nifedipine and nitrendipine (internal standard) are separated on a C18 column with a mobile phase consisting of acetonitrile-13 mM phosphate buffer pH 7 (65:35, v/v) followed by UV detection at 338 nm. Validation of the method demonstrated good recoveries (>90%), sensitivity (limit of quantification, 2 ng/ml), based on a 500 microl sample volume, accuracy and precision (<5.5% in concentrations greater than the limit of quantitation). This methodology has been used for bioequivalence studies.
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