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  • Title: A simple approach for the simultaneous isolation and immobilization of invertase using crude extracts of yeast and Jack bean meal.
    Author: Melo JS, D'Souza SF.
    Journal: J Biochem Biophys Methods; 2000 Mar 16; 42(3):133-5. PubMed ID: 10737219.
    Abstract:
    Crude cell-free extract of yeast cells was mixed with sufficient amount of Jack bean meal extract so as to precipitate all the invertase. The precipitate was then cross-linked using 2% glutaraldehyde retaining over 60% of the activity. The immobilized invertase could be reused for over ten batches without loss in activity.
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