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  • Title: The iteron regions necessary for the RepE-iteron interaction in vivo in mini-F plasmids of Escherichia coli.
    Author: Uga H, Komori H, Miki K, Wada C.
    Journal: J Biochem; 2000 Apr; 127(4):537-41. PubMed ID: 10739943.
    Abstract:
    We have determined the nucleotide positions of an incC iteron essential for RepE binding by analyzing mutated incC iterons defective in exerting incompatibility towards mini-F plasmids. The mutations affecting this incompatibility occurred mostly at two positions within the incC iteron, i.e. an iteron conserved position and a mini-F specific position. Most of the iterons with a base-change at either of these two positions had lost the binding affinity for RepE. This agrees with the crystallographic structure of the RepE-iteron complex which showed that the N and C terminal domains of RepE interact with the two major grooves on one face of the iteron DNA. These grooves contain the iteron conserved and mini-F specific positions necessary for RepE binding. Thus the binding mode may be common to in the case of mini-F like plasmids.
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