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  • Title: Development of a combined PCR-culture technique for the rapid detection of Arcobacter spp. in chicken meat.
    Author: González I, García T, Antolín A, Hernández PE, Martín R.
    Journal: Lett Appl Microbiol; 2000 Mar; 30(3):207-12. PubMed ID: 10747252.
    Abstract:
    A combined PCR-culture technique was developed to detect Arcobacter spp. in fresh chicken meat. Following a short selective enrichment of chicken samples, bacterial DNA was extracted and amplified using primers targeted at the genes encoding 16S rRNA of Arcobacter spp. The selected primers amplify a 181-bp fragment from all Arcobacter spp., whereas no PCR product is generated for other bacteria, including the closely related Campylobacter and Helicobacter species. The assay was used to screen 96 retail-purchased chicken samples for the presence of Arcobacter spp. Fifty-three percent of the samples analysed were positive for this micro-organism. The assay is simple and sensitive and reduces the amount of time required to positively detect Arcobacter spp. in poultry meat.
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