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  • Title: Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals.
    Author: Tsakiris S, Angelogianni P, Schulpis KH, Stavridis JC.
    Journal: Clin Biochem; 2000 Mar; 33(2):103-6. PubMed ID: 10751587.
    Abstract:
    OBJECTIVE: To investigate whether the preincubation of brain homogenates with L-phenylalanine (Phe) could reverse the free radical effects on brain acetylcholinesterase (AChE) activity, since it has been reported that Phe binds hydroxyl radicals ((*)OH). DESIGN AND METHODS: Two well established systems were used for production of free radicals: (a) FeSO(4) (84 microM) plus ascorbic acid (400 microM), and (b) FeSO(4), ascorbic acid and H(2)O(2) (1 mM) at 37 degrees C in homogenates of adult rat whole brain. Changes in brain AChE activity were studied in the presence of each system separately. RESULTS: AChE was inhibited (18-28%) by both systems of free radicals. This inhibition was reversed when the brain homogenate was preincubated with Phe 1.8 mM. CONCLUSIONS: In accordance with our previous reports, Phe could protect against the direct action of (*)OH radicals on brain AChE and in this way it might be useful in the prevention of certain cholinergic neural dysfunctions.
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