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  • Title: [Restriction endonucleases from various bacterial strains exhibit an ice-nucleating activity].
    Author: Pertsev AV, Den'mukhametov MM, Anoshkin AG, Ariskina EV, Berezin IA, Solonin AS, Kuz'min NP.
    Journal: Prikl Biokhim Mikrobiol; 2000; 36(1):13-6. PubMed ID: 10752077.
    Abstract:
    Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions. Endonucleases Pfl211I, Psp8I, and Psp23I were isolated and purified from two Pseudomonas sp. strains and a Pseudomonas fluorescens strain. Restriction endonucleases Pfl21I and Psp23I were shown to recognize and cleave the DNA nucleotide sequence 5'-CTGCA decrease G-3'. Endonuclease Psp8I recognized and cleaved the DNA nucleotide sequence 5'-G decrease GATCC-3'. These endonucleases were found to be true isoschizomers of PstI and BamHI, respectively.
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