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  • Title: The rug3 locus of pea encodes plastidial phosphoglucomutase.
    Author: Harrison CJ, Mould RM, Leech MJ, Johnson SA, Turner L, Schreck SL, Baird KM, Jack PL, Rawsthorne S, Hedley CL, Wang TL.
    Journal: Plant Physiol; 2000 Apr; 122(4):1187-92. PubMed ID: 10759514.
    Abstract:
    Two cDNA clones were isolated from pea (Pisum sativum L.) and their deduced amino acid sequences shown to have significant homology to phosphoglucomutases from eukaryotic and prokaryotic sources. The longer cDNA contained a putative transit-peptide-encoding sequence, supporting the hypothesis that the isolated clones represent the cytosolic and plastidial isoforms of phosphoglucomutase in pea. Plastid protein import assays confirmed that the putative plastidial isoform was targeted to the plastid stroma where it was proteolytically processed. Expression, co-segregation, linkage, and molecular analyses have confirmed that the rug3 locus of pea encodes plastidial phosphoglucomutase. Mutations at this locus result in a near-starchless phenotype of the plant.
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