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  • Title: New positive selection system based on the parD (kis/kid) system of the R1 plasmid.
    Author: Gabant P, Van Reeth T, Drèze PL, Faelen M, Szpirer C, Szpirer J.
    Journal: Biotechniques; 2000 Apr; 28(4):784-8. PubMed ID: 10769758.
    Abstract:
    The use of vectors that are designed to allow positive selection of recombinants facilitates cloning experiments in E. coli. Using kid, a lethal gene of the R1 plasmid parD locus, we generated pKID vectors leading to high selective efficiency of recombinants (greater than 90%). The E. coli bacterial host used to propagate these vectors produces the Kis protein, the natural antagonist of Kid. This new positive-selection system exhibits the same efficiency as the original ccdB-based selection vectors, pKIL (4). We also show that the ccdB and kid systems are independent. This property increases the potential of plasmidic poison-antidote systems for genetic applications and opens the door to a generation of new vectors containing the two selection systems.
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