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  • Title: Voltammetry on mercury and carbon electrodes as a tool for studies of metallothionein interactions with metal ions.
    Author: Sestáková I, Mader P.
    Journal: Cell Mol Biol (Noisy-le-grand); 2000 Mar; 46(2):257-67. PubMed ID: 10774918.
    Abstract:
    Rabbit liver Cd-metallothionein (CdMT) and Cd-complex of synthetically prepared pentapeptide (gamma-Glu-Cys)2-Gly were studied as examples of animal and plant metallothioneins. Using hanging mercury electrode, cathodic stripping voltammetry after adsorptive accumulation of the Cd(II)-SR complex at different potentials, is suitable for estimating changes occurring in metal coordination due to the presence of metal ions such as Zn2+, Cu2+, Hg2+ or excessive Cd2+. Conditions under which similar behaviour can be observed for both CdMT and Cd-pentapeptide complex are specified. On carbon electrodes, detailed study of reduction processes of Cd(II)-SR complexes is prevented by occurrence of a large catalytic current; oxidation processes are more suitable for study at these electrodes. Carbon composite paste electrode (10% SiO2) allows deposition of Cd(II)-SR complex during its reduction, as was demonstrated with Cd-cysteine, CdMT or Cd-pentapeptide complex. After deposition, oxidation peak of the uncomplexed Cd2+ ions and one or two oxidation peaks corresponding to a formation of the RS-Cd(II) complex are observed. Also, similarly as on Hg electrode, it was observed that excessive Cd2+ or Zn2+ ions influence oxidation peaks of the RS-Cd(II) complex formation. Combination of measurements on mercury electrode and composite paste electrode is recommended for studies of metallothionein interactions with metal ions or other metal complexes.
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