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  • Title: [Analysis of log-normal components of fluorescence spectra of prodan and acrylodan bound to proteins].
    Author: Emel'ianenko VI, Reshetniak IaK, Andreev OA, Burshteĭn EA.
    Journal: Biofizika; 2000; 45(2):207-19. PubMed ID: 10776530.
    Abstract:
    Steady-state fluorescence spectra of prodan and acrylodan covalently bound to cystein residue of Lys-Cys-Phe tripeptide in solvents of different polarity were analyzed. It was shown that the shape of spectral bands is well described by a log-normal function. Linear relations between three shape-determining parameters of the log-normal function (namely, the positions of spectral maximum and two half-maximum amplitudes) were revealed and evaluated for both fluorophores. This finding enabled us to present the shape of spectral bands of these fluorophores in any environment as analytical log-normal functions depending on only two parameters, the maximum position and the peak amplitude. The empirical uniparametric log-normal curve was used for the analysis of composite fluorescence spectra of prodan bound to bovine serum albumin and acrylodan covalently attached to actin or subfragment 1 of myosin.
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