These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Motion determination in actin filament fluorescence images with a spatio-temporal orientation analysis method.
    Author: Uttenweiler D, Veigel C, Steubing R, Götz C, Mann S, Haussecker H, Jähne B, Fink RH.
    Journal: Biophys J; 2000 May; 78(5):2709-15. PubMed ID: 10777767.
    Abstract:
    We present a novel approach of automatically measuring motion in series of microscopic fluorescence images. As a differential method, the three-dimensional structure tensor technique is used to calculate the displacement vector field for every image of the sequence, from which the velocities are subsequently derived. We have used this method for the analysis of the movement of single actin filaments in the in vitro motility assay, where fluorescently labeled actin filaments move over a myosin decorated surface. With its fast implementation and subpixel accuracy, this approach is, in general, very valuable for analyzing dynamic processes by image sequence analysis.
    [Abstract] [Full Text] [Related] [New Search]