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  • Title: [Transformation of Mycoplasma hominis by plasmid pAM120 using electroporation].
    Author: Aleksandrova NM, Bevova MR, Govorun VM.
    Journal: Genetika; 2000 Mar; 36(3):309-13. PubMed ID: 10779903.
    Abstract:
    Mycoplasma hominis was transformed by electroporation with plasmid pAM120 containing the transposon Tn916 that carried the tetM gene responsible for the resistance to tetracycline. The frequency of transformation was 10(-7)-10(-8) colony-forming units (CFU) per 10 micrograms of plasmid DNA. The PCR analysis of transformed DNA confirmed the transposon integration into the mycoplasma genome.
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