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  • Title: Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization.
    Author: Sghir A, Gramet G, Suau A, Rochet V, Pochart P, Dore J.
    Journal: Appl Environ Microbiol; 2000 May; 66(5):2263-6. PubMed ID: 10788414.
    Abstract:
    To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed, validated, and used with a set of five 16S rRNA-targeted oligonucleotide probes. Application of the six probes to fecal samples from 27 human adults showed additivity of 70% of the total 16S rRNA detected by the bacterial domain probe. The Bacteroides group-specific probe accounted for 37% +/- 16% of the total rRNA, while the enteric group probe accounted for less than 1%. Clostridium leptum subgroup and Clostridium coccoides group-specific probes accounted for 16% +/- 7% and 14% +/- 6%, respectively, while Bifidobacterium and Lactobacillus groups made up less than 2%.
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