These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The effects of granulocyte colony stimulating factor on chemiluminescence and lipid peroxidation of blood platelets treated with cisplatin.
    Author: Olas B, Wachowicz B, Buczyński A.
    Journal: Anticancer Drugs; 2000 Feb; 11(2):79-84. PubMed ID: 10789589.
    Abstract:
    The effects of granulocyte colony stimulation factor (G-CSF) at concentrations of 0.08, 0.8 and 8 microg/ml on reactive oxygen species (ROS) generation and lipid peroxidation induced by cisplatin in pig blood platelets were investigated. The level of reactive oxygen species (O2, H2O2, singlet oxygen and organic radicals) generated in platelets was measured by the chemiluminescence method. Lipid peroxidation was determined by the thiobarbituric acid technique and was expressed as thiobarbituric acid reactive substances. G-CSF at the concentration of 0.08 microg/ml had a strong inhibitory effect (about 60% inhibition) on the production of ROS in the isolated pig platelets. This cytokine also significantly reduced lipid peroxidation in control platelets and platelets treated with cisplatin (p<0.05). In the presence of G-CSF in the incubation medium (0.8 microg/ml) cisplatin-induced generation of ROS was also reduced (p<0.05). This study demonstrates that G-CSF has a protective effect against the oxidative stress in blood platelets caused by cisplatin.
    [Abstract] [Full Text] [Related] [New Search]