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  • Title: Trials of rosette test application for determination of immune response to antigen D from the Rh system in blood donors immunized with human erythrocytes.
    Author: Maślanka K.
    Journal: Arch Immunol Ther Exp (Warsz); 1978; 26(1-6):1105-9. PubMed ID: 107911.
    Abstract:
    Periodic control examinations of Rh-negative blood donors sensitized with human Rh-positive erythrocytes were performed to establish the differences in the levels of T and B lymphocytes and their correlation with the production of anti-Rh D antibodies. The investigations were carried out on 10 healthy blood donors in the initial period of immunization and 8 blood donors who had received multiple injections of these erythrocytes and who were producing anti-Rh antibodies. The control group included 10 non-immunized blood donors. The percentage of T and B lymphocytes was calculated on the basis of the rosette tests (E, EA, EAC) while the level of antibodies produced in serum was determined by serological methods. During immunization of blood donors no significant differences were observed in the level of T lymphocytes, while the level of B lymphocytes increased, especially in the 3rd week after the 1st or 2nd injection of erythrocytes and this was not correlated with the production of anti-Rh D antibodies in later period.
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