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  • Title: Heat-shock tagging: a simple method for expression and isolation of plant genome DNA flanked by T-DNA insertions.
    Author: Matsuhara S, Jingu F, Takahashi T, Komeda Y.
    Journal: Plant J; 2000 Apr; 22(1):79-86. PubMed ID: 10792823.
    Abstract:
    This paper describes expression profiles of the Arabidopsis HSP18.2 heat-shock gene promoter by using three different reporter genes, and the application of this promoter to a method we have developed to drive heat-shock-dependent transcription of plant genome DNA flanked by T-DNA insertions. We show that, irrespective of the location of the T-DNA insertion, an HSP18.2 promoter towards the left border of the T-DNA effectively induces transcription of flanking genome sequences in response to heat shock. If polyadenylated, tagged transcripts can be easily identified by RT-PCR.
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