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  • Title: A simultaneous method for detection and quantitation of T- and B-lymphocytes.
    Author: Robinson JA, Lertratanakul Y.
    Journal: J Immunol Methods; 1975; 8(1-2):53-60. PubMed ID: 1080176.
    Abstract:
    Assay for quantitation of B- and T--lymphocyted from small volumes of heparinized man blood has been developed by simultaneously reacting Ficoll-Hypaque purified mononuclear cells with antobody-coated chicken erythrocytes to for rosettes with B-lymphocytes, and sheep erythrocytes which formed non-immune rosettes with Tlymphocytes. Distribution of various lymphocyte populations in normals are T-cells, 60.9+/-7%, B-cells 16.4+/-4%, and lymphocytes with both receptors, 1.6+/-0.6%.
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