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  • Title: [Use of RT-PCR and nested PCR for diagnosing enterovirus infections].
    Author: Binduga-Gajewska I, Gut W, Wielkopolska A, Jarzabek Z.
    Journal: Med Dosw Mikrobiol; 1999; 51(3-4):375-81. PubMed ID: 10803267.
    Abstract:
    An application of reverse transcription (RT-PCR) and nested-PCR for the detection of human enteroviruses isolated in Poland is described. A specific 297-bp fragment was amplified by the method from all studied enteroviruses strains. Amplification system and the nested primers allowed to detect RNA isolated from 10(1) TCID50/ml which corresponded to one molecule of viral RNA in tested volume. However, applicability of the method for routine diagnosis of enterovirus neuro-infection should be reassessed in extensive clinical studies.
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