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  • Title: [Experimental study of apoptosis induced by cisplatin in human ovarian carcinoma AO 10/17 cells].
    Author: Liu Q, Chen W, Li X.
    Journal: Zhonghua Fu Chan Ke Za Zhi; 1998 Jul; 33(7):422-4. PubMed ID: 10806704.
    Abstract:
    OBJECTIVE: Whether apoptosis induced by cisplatin resulted in tumor growth inhibition and the relationships between apoptosis and cell cycle, intracellular and extracellular Ca++ concentration were investi- gated. METHODS: AO 10/17 cells were treated with cisplatin. Morphological changes (HE stain) were observed under light microscope; DNA degradation was assayed by electrophoresis on agarose gel. The change of cell cycle was analyzed by flow cytometer and cytosolic Ca++ concentrations were monitored using probe of calcium ion fluo-3/AM. RESULTS: Under the influence of cisplatin, AO 10/17 cells exhibited changes of apotosis both in morphology and in the characteristics of electrophoresis. Meanwhile the cell cycle also showed special change and intracellular Ca++ concentration was increased. Increase in extracellular Ca++ concentration when AO 10/17 cells were treated with CaCl2 and cisplatin did not enhance the efficacy of cisplatin. CONCLUSION: The results suggest that apoptosis followed by change of cytosolic Ca++ concentration is one of the mechanism of cisplatin in inhibition of ovarian tumor growth. It is closely related to cell cycle.
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