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  • Title: Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease.
    Author: Ruggeri ZM, Mannucci PM, Jeffcoate SL, Ingram GI.
    Journal: Br J Haematol; 1976 Jun; 33(2):221-32. PubMed ID: 1083743.
    Abstract:
    A solid phase non-competitive immunoradiometric assay (IRMA) has been developed which allows measurement of factor VIII related antigen (VIIIR:AG) levels in normal plasma as low as 2.5 x 10(-4) U/ml. The assay is based on the extraction of VIIIR:AG from test plasma by means of polystrene tubes coated with a specific unlabelled anti-VIIIR:AG rabbit antiserum and subsequent labelling of the extracted antigen with 125I-labelled anti-VIIIR:AG rabbit IgG.
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