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  • Title: Expression and purification of woodchuck tumour necrosis factor alpha.
    Author: Lohrengel B, Lu M, Bauer D, Roggendorf M.
    Journal: Cytokine; 2000 Jun; 12(6):573-7. PubMed ID: 10843731.
    Abstract:
    The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-alpha) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-alpha was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-alpha was purified and used for the production of neutralizing antisera.
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