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  • Title: alpha-Amylase production by free and immobilized Bacillus subtilis.
    Author: Duran-Paramo E, Garcia-Kirchner O, Hervagault JF, Thomas D, Barbotin JN.
    Journal: Appl Biochem Biotechnol; 2000; 84-86():479-85. PubMed ID: 10849814.
    Abstract:
    The effect of glucose on the alpha-amylase production by Bacillus subtilis ATCC-21556 was studied. Initial glucose concentrations up to 20 g/L were found to be directly proportional to the specific alpha-amylase production in an immobilized-cell batch system, whereas a free-cell batch system presented an inversely proportional relationship with the initial glucose concentration. This might be owing to the alpha-amylase repression by the glucose present in the culture medium. Three hundred eighty-five percent of the specific alpha-amylase production with the free-cell system was produced by the immobilized-cell batch culture.
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