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  • Title: A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 2: competitive binding.
    Author: Park SB, Reedy BM, Standaert RF.
    Journal: Bioorg Med Chem Lett; 2000 May 01; 10(9):955-6. PubMed ID: 10853667.
    Abstract:
    A qualitative assay for the evaluation of soluble ligands of the nuclear import receptor karyopherin alpha has been developed. The assay relies on competition with an immobilized ligand, the nuclear localization signal (NLS) from nucleoplasmin, for binding to the receptor, which is detected by an enzyme-linked colorimetric method.
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