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  • Title: Importance of holotoxin assembly in Ptl-mediated secretion of pertussis toxin from Bordetella pertussis.
    Author: Farizo KM, Huang T, Burns DL.
    Journal: Infect Immun; 2000 Jul; 68(7):4049-54. PubMed ID: 10858221.
    Abstract:
    We examined the structural components of pertussis toxin that are required for efficient export from Bordetella pertussis via the Ptl system, a member of the type IV family of macromolecular transporters. First, we constructed a strain of B. pertussis that contains a functional Ptl system but does not produce pertussis toxin. Plasmids which express either the S1 subunit or the B oligomer were then introduced into this strain. We found that the B oligomer of the toxin is not secreted in the absence of the S1 subunit. Conversely, the S1 subunit is also not secreted by a Ptl-mediated mechanism in the absence of the B oligomer. Thus, an assembled holotoxin is required for Ptl-mediated export of pertussis toxin from B. pertussis.
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