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  • Title: [Effect of lipids on I and III procollagen mRNA expression of hepatic stellate cells].
    Author: Lu L, Zeng M, Li J.
    Journal: Zhonghua Gan Zang Bing Za Zhi; 2000 Apr; 8(2):73-5. PubMed ID: 10861106.
    Abstract:
    OBJECTIVE: To study the effect of lipids on I and III procollagen mRNA expression of hepatic stellate cells (HSC). METHODS: HSC were isolated and cultured from the liver of Wistar rats by in situ perfusion with pronase and collagenase and the density gradient centrifugation with Nycodenz. HSC were incubated with triglyceride (25mg/L) and very low-density lipid (VLDL, 25 mg/L) for 10d, respectively. I and III procollagen mRNA expression of HSC was detected by Northern blot hybridization. RESULTS: After HSC was stimulated with triglyceride and VLDL, the expression of I and III procollagen mRNA increased obviously. CONCLUSION: Lipids may promote the expression of I and III procollagen mRNA, and may be associated with fatty liver and hepatic fibrogenesis.
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