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  • Title: Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus.
    Author: Gangemi JD, Sharp DG.
    Journal: J Virol; 1976 Oct; 20(1):319-23. PubMed ID: 1086369.
    Abstract:
    A restriction endonuclease from Haemophilus influenzae (Hind III) specifically cleaved vaccinia DNA into 14 fragments. The molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). Hind III digestion of the DNA from the WR and CV-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. The average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6).
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