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  • Title: Leukocyte adherence contributes to disruption of the blood-brain barrier during activation of mast cells.
    Author: Mayhan WG.
    Journal: Brain Res; 2000 Jun 30; 869(1-2):112-20. PubMed ID: 10865065.
    Abstract:
    The goal of this study was to examine the role of leukocytes in disruption of the blood-brain barrier during activation of mast cells using compound 48/80. We examined the pial microcirculation in rats using intravital fluorescence microscopy. Permeability of the blood-brain barrier (clearance of fluorescent-labeled dextran; molecular weight 10000 daltons; FITC-dextran-10 K) was determined while suffusing with vehicle or compound 48/80 (10 or 25 microg/ml). During superfusion with vehicle (saline), clearance of FITC-dextran-10 K from pial vessels was modest and remained relatively constant during the experimental period (0.52+/-0.05 ml/sx10(-6) at 80 min). In addition, diameter of pial arterioles remained constant (32+/-5 microm) while suffusing with vehicle. In contrast, topical application of compound 48/80 produced marked disruption of the blood-brain barrier to FITC-dextran-10 K. For example, suffusion with compound 48/80 (25 microg/ml) increased clearance of FITC-dextran-10 K about 4-fold to 2.26+/-0.25 ml/sx10(-6) at 80 min. In addition, superfusion with compound 48/80 (25 microg/ml) constricted pial arterioles by 26+/-9% at 80 min. To determine a potential role for leukocyte adhesion to endothelium in disruption of the blood-brain barrier during suffusion with compound 48/80, we examined permeability during suffusion with compound 48/80 (25 microg/ml) in the presence of WT.3 (2 mg/kg i.v.), a monoclonal antibody directed against the functional epitope of the leukocyte adhesive glycoprotein (CD18; LFA-1beta). We found that infusion of WT.3 markedly attenuated disruption of the blood-brain barrier to FITC-dextran-10 K in response to compound 48/80. The clearance of FITC-dextran-10 K during superfusion with compound 48/80 in the presence of WT.3 was 1.29+/-0.14 ml/sx10(-6) at 80 min (P<0.05). Thus, the findings of the present study suggest that application of compound 48/80, to degranulate mast cells, activates the adhesion of leukocytes to cerebral venular endothelium which contributes to disruption of the blood-brain barrier.
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