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  • Title: [Noncapsular mutants of Vibrio cholerae serogroup O139: their isolation, identification and use for the preparation of an O139 diagnostic antiserum].
    Author: Chekhovskaia GV, Shchelkanova EIu, Smirnova NI.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 2000; (1):34-7. PubMed ID: 10876889.
    Abstract:
    On the basis of V. cholerae strain P16064, serogroup O139, spontaneous and transposon mutants with the stable lose of the capacity for producing the polysaccharide capsule, but retaining antigen O139, have been obtained. As revealed in this study, capsular and noncapsular strains differ in their sensitivity to cholera phages 20 and Inaba, as well as in agglutination with O139-antiserum. These data make it possible to use of bacteriophages for the differentiation of capsular and noncapsular strains. The use of noncapsular mutants ensure obtaining rabbit O139-antisera with higher antibody titer.
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