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  • Title: A simple method for measurement of CD14weak CD16strong monocytes in peripheral blood.
    Author: Hübl W, Ziegler-Heitbrock LH, Bayer PM.
    Journal: Immunobiology; 2000 May; 202(1):2-10. PubMed ID: 10879683.
    Abstract:
    Mounting evidence for the clinical significance of the CD 14weak CD16strong monocyte subpopulation in peripheral blood induced the demand for an efficient method for its determination. We propose a simple, fast, no-wash flow cytometric method using fluorescence-labelled anti-CD14, anti-CD16, and anti-HLA-DR antibodies and ammonium chloride-based erythrocyte lysis. This type of analysis can be performed on a standard three-colour flow cytometer. The method avoids interference by NK-cells and neutrophil granulocytes without defining monocytes by stringent light scatter criteria that might lead to a loss of CD14weak CD16strong monocytes. It, therefore, offers high reliability and accuracy. Its performance recommends the method to be used for routine clinical measurements of CD14weak CD16strong monocytes.
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