These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Fractionation of human blood lymphocytes on Helix pomatia A haemagglutinin coupled to sepharose beads.
    Author: Hellström U, Hammarström S, Dillner ML, Perlmann H, Perlmann P.
    Journal: Scand J Immunol; 1976 Jun; Suppl 5():45-55. PubMed ID: 1088709.
    Abstract:
    Treatment of human blood lymphocytes with neuraminidase has previously been shown to uncover receptors for the A haemagglutinin of the snail Helix pomatia (HP). Neuraminidase-treated lymphocytes were now fractionated on columns charged with large Sepharose particles to which HP had been coupled covalently. HP-receptor negative (HP-) lymphocytes passed the columns while HP-receptor positive (HP+) lymphocytes were retained. The latter cells were eluted by addition of the competitive hapten N-acetyl-D-galactosamine (D-GalNAc). The total yield of cells recovered after fractional was 60-80% Surface marker studies indicated that there was no selective loss of any of the major lymphocyte subpopulations. The fraction that passed the columns (fraction I) consisted of approximately 10% of all lymphocytes. It contained approximately 1% HP+cells and approximately 3% of all lymphocytes forming rosettes which sheep erythrocytes (E+ cells) present before fractionation. 50-55% of the lymphocytes in this fraction had surface-bound immunoglobulin (SIg+ cells) and complement receptors (EAC+ cells). Of the SIg+ cells, approximately 60% were true B cells while the remaining 40% had IgG adsorbed to their surface. The majority of the B cells were recovered in this fraction. The lymphocytes of this fraction responded poorly to T-cell mitogen but had an enhanced K-cell activity to chicken erythrocytes. Elution of the cells retained on the column with 0.1 mg/ml D-GalNAc gave a fraction II, consisting of approximately 15% of all lymphocytes. This fraction had a mixed composition. The majority of the cells (approximately 45%) were recovered by subsequent elution with 1.0 mg/ml D-GalNAc. This fraction III was strongly enriched with HP+ and E+ cells (T cells). About 10% of the HP+ cells in this fraction were SIg+. However, on the majority of these cells this surface-bound immunoglobulin was probably externally absorbed IgG. These HP+-SIg" cells were also EAC+ and had Fc receptors, as shown by rosette formation with IgG-coated bovine erythrocytes. The lymphocytes of fraction III responded most strongly to T-cell mitogen while their K-cell activity was weak.
    [Abstract] [Full Text] [Related] [New Search]