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  • Title: Reactivation of heterodimer and individual subunits of penicillin acylase from E. coli after inactivation in urea.
    Author: Shamolina TA, Svedas VK.
    Journal: Biochemistry (Mosc); 2000 Jun; 65(6):672-6. PubMed ID: 10887285.
    Abstract:
    Individual subunits of penicillin acylase from E. coli were isolated by gel-filtration under denaturing conditions (8 M urea). Recovery of the catalytic activity of the penicillin acylase heterodimer was studied after removal of urea. In the case of the heterodimer, 40-60% of the initial activity was recovered, whereas the activity of individual subunits was not recovered. Combination of native enzyme subunits with subunits isolated from the enzyme pre-inactivated with the irreversible inhibitor phenylmethylsulfonyl fluoride resulted in heterodimers which were active only in the case of involvement of the beta-subunit of the active enzyme.
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