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  • Title: Cloning and recombinant expression of the La RNA-binding protein from Trypanosoma brucei.
    Author: Westermann S, Weber K.
    Journal: Biochim Biophys Acta; 2000 Jul 24; 1492(2-3):483-7. PubMed ID: 10899586.
    Abstract:
    We report the isolation, cloning and recombinant expression of a Trypanosoma brucei homolog of the La RNA-binding protein. Based on peptide sequence information we have isolated a cDNA clone which encodes a protein of 335 amino acids with a predicted molecular weight of 37.7 kDa. The amino acid sequence fits the domain structure of known La proteins and contains a putative ATP-binding site located in the COOH-terminal domain. The cDNA was expressed as a glutathione S-transferase fusion protein in Escherichia coli, and the recombinant protein displayed RNA-binding activity in an electrophoretic mobility shift assay.
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