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  • Title: Is endogenous isoprene the only coeluting compound in the measurement of breath pentane?
    Author: Mitsui T, Naitoh K, Tsuda T, Hirabayashi T, Kondo T.
    Journal: Clin Chim Acta; 2000 Sep; 299(1-2):193-8. PubMed ID: 10900304.
    Abstract:
    Pentane is a widely used index of lipid peroxidation. Although isopentane, an isomer of pentane, is a major component of ambient air in urban areas, many studies have disregarded the possibility that this compound is coeluted in the measurement of breath pentane. In the present study, a gas chromatograph equipped with a cold trap apparatus and a large-bore glass capillary column was used for determination of pentane, isopentane and isoprene in breath and ambient air. Isoprene was detected in all subjects at a concentration higher than that in the ambient air. However, the concentrations of breath pentane and isopentane were similar to, or less than, those of the ambient air. We suggest that great care is required in the measurement of breath pentane so that endogenous isoprene and ambient isopentane are not coeluted.
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