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  • Title: Simultaneous determination of alpha-tocopherol and beta-carotene in olive oil by reversed-phase high-performance liquid chromatography.
    Author: Gimeno E, Calero E, Castellote AI, Lamuela-Raventós RM, de la Torre MC, López-Sabater MC.
    Journal: J Chromatogr A; 2000 Jun 09; 881(1-2):255-9. PubMed ID: 10905709.
    Abstract:
    A reversed-phase high-performance liquid chromatographic method was developed for the determination, in one run, of alpha-tocopherol and beta-carotene in virgin olive oil. The method involved a rapid saponification and a later extraction with a mixture of hexane-ethyl acetate. The chromatographic system consists of an ODS-2 column with a mobile phase of methanol-water-butanol and a diode-array detector. Linearity, precision, recovery and sensitivity were satisfactory. The main advantage of the proposed method is the speed and simultaneous determination of both compounds at the same time.
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