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  • Title: Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase.
    Author: Anthony LC, Artsimovitch I, Svetlov V, Landick R, Burgess RR.
    Journal: Protein Expr Purif; 2000 Aug; 19(3):350-4. PubMed ID: 10910724.
    Abstract:
    Bacillus subtilis core RNA polymerase, containing a His(6)-fusion to the C-terminus of the beta' subunit, was isolated by Ni-NTA, Superdex 200 gel filtration, and Mono Q anion-exchange chromatography. The purified core enzyme was shown to be free of the major sigma factor(A) and the transcription factors NusA and GreA. The purification procedure can be completed within 1 working day, is scalable, and yields highly purified and active core RNA polymerase.
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