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  • Title: [Antitumor effect of M-CSF and/or IFN-gamma gene cotransfected-macrophages by intratumoral injection].
    Author: Lei H, Cao X, Yu Y.
    Journal: Zhonghua Zhong Liu Za Zhi; 1998 May; 20(3):174-7. PubMed ID: 10920999.
    Abstract:
    OBJECTIVE: To investigate the in vivo antitumor effects of M-CSF and/or IFN-gamma gene transfected-macrophages and their related immunological mechanisms. METHODS: Survival time and antitumor effects in vivo of tumor-bearing mice treated with gene-transfected macrophages were observed. NK cell and CTL activity was detected with 4 hr 51Cr release assay, cytotoxicity of macrophages was detected with indirect MTT methods, activity of IL-2, TNF, IFN-gamma were detected by routine methods. RESULTS: 25% of the tumor-bearing mice after treatment with IFN-gamma gene-transfected, M-CSF and IFN-gamma gene co-transfected macrophages survived more than 3 month. The CTL and macrophage activity of the tumor-bearing mice was enhanced. Levels of TNF, IL-2, IFN-gamma in the supernatants of splenocytes induced in vitro were also increased. Histologic analyses of the tumor tissue after treatment with M-CSF and/or IFN-gamma gene-transfected macrophages showed a large amount of lymphocyte infiltration. CONCLUSION: IFN-gamma and M-CSF gene co-transfection into macrophages are proved to enhance their antitumor potential efficiently through direct augmentation of their tumoricidal cytotoxicity and induction of host antitumor immunity.
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