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  • Title: Evaluation of LightCycler PCR for implementation of laboratory diagnosis of herpes simplex virus infections.
    Author: Espy MJ, Ross TK, Teo R, Svien KA, Wold AD, Uhl JR, Smith TF.
    Journal: J Clin Microbiol; 2000 Aug; 38(8):3116-8. PubMed ID: 10921993.
    Abstract:
    Five hundred specimens (288 genital, 192 dermal, and 20 ocular) were extracted by technologists, and the DNA was assayed by LightCycler PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by conventional tube and shell vial cell culture. One hundred fifty-eight confirmed (by cell culture and TK target PCR) positive and LightCycler-positive specimens were detected during the first 30 PCR cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK). LightCycler PCR is more sensitive (n = 197; 23.1%) than cell cultures (n = 150) for the routine laboratory detection of herpes simplex virus infections.
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